MAGNETIC RESONANCE ANGIOGRAPHY (MRA)
A Brief Overview

Greg Brown SMRT Royal Adelaide Hospital
Prepared in September 1997 for a general audience familiar with basic MRI principles

  • Introduction
  • Factors Affecting the Appearance of flowing Blood
  • Important Characteristics of Flow
  • Time of Flight MRA (TOF)
  • TOF Applications
  • Phase Contrast MRA (PCA)
  • When is MRA Appropriate?

  •  
  • Summary of Basic MRA
  • Flow Quantification and Contrast Enhanced MRA
  • Flow Quantification MR
  • Contrast Enhanced MRA (GD-MRA)
  • Contrast Enhanced MRA Technique

  • Suggested Readings 
    References
    Back to Greg's MRI Page Contact the Author


    Introduction
    Flow effects in MRI can produce a range of artefacts. For example fast moving blood produces flow voids, blood flowing in to the outer slices of an imaging volume produces high signals, pulsatile flow creates ghost images of the vessel extending across the image in the phase encoding direction. MRA has developed by taking advantage of these "artefacts" to create predictable image contrast due to the nature of flow.

    At first encounter MRA images look like a conventional digital subtraction angiogram (DSA) but the similarity is an illusion. When interpreting the images it's important to remember how they are created, what mechanisms create the image contrast and how these methods can emphasize or hide different vascular disease processes.

    MR angiograms are usually processed or reconstructed images derived from a set of slices referred to as the "base images". The base images can be acquired sequentially or concurrently with each method being subject to different artefacts. Image resolution can be high in plane (less than 1 mm.), but lower in the slice thickness direction (1-3 mm.) and as a result views at greater angles to the original slice plane will demonstrate step artefacts.

    MRA image contrast is primarily created by flow, rather than displaying the vessel lumen. MRA shows blood as it travels as distinct from the lumen it is travelling in. If there is no flow MRA wont show the vessel. Technical parameters of the MRA sequence greatly affect the sensitivity of the images to flow with different velocities or directions, turbulent flow and vessel size. top of page



     
    Factors Affecting the Appearance of Flowing Blood
    Characteristics of Flow Pulse Sequence Parameters
    Velocity TR Repetition Time 
    Acceleration TE Echo Time
    Pulsation Slice Thickness
    Vortices Flip Angle
    Distribution of velocities 
    across vessel
    Flow Compensation
    Flow direction Saturation Pulses
    Cardiac Phase Slice Location within Acquired Volume
    T1 of Blood 
    (can be altered by Gd)
    Slice Acquisition Strategy
    (2D single slice ,2D multi-slice, 3DFT)
      Cardiac Triggering
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    Important Characteristics of Flow
    flow map in bifurcation

    MRA images optimally display areas of constant flow velocity, but there are many situations where the flow within a voxel has non-uniform speed or direction. The diagram opposite illustrates the streamlines of flow in the normal carotid bifurcation showing regions of reversed and crossed flow. In a diseased vessel these patterns are even more complex. Similar loss of streamline flow occurs at all vessel junctions and stenoses, and in regions of mural thrombosis. It results in a loss of signal, due to the loss of phase coherence between spins in the voxel. This effect must be considered when interpreting images or selecting technique. This signal loss, usually only noticeable distal to a stenosis, used to be an obvious characteristic of MRA images. It is now minimized by using small voxels and the shortest possible TE. Signal loss from disorganized flow is most noticeable in TOF imaging but also affects the PCA images.

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    Time Of Flight MRA (TOF)

    TOF is the most widely used MRA technique. It provides robust images for a range of clinical questions. TOF use short TR sequences acquired as slices perpendicular to the direction of blood flow. The short TR period saturates stationary tissue driving its MR signal down. By contrast the flow related enhancement (FRE) effect yields a high signal from blood moving into the slice making the vessels stand out bright against a grey background. Maximum flow signal is achieved when a totally new column of blood enters the slice every TR period.

    Maximum intensity when flow velocity > Slice Thickness/TR.

    This relationship makes the technique sensitive to flow velocity. TR and slice thickness must be appropriate to the flow velocities expected and even small changes in slice thickness can affect the performance of the MRA sequence. Saturation bands can be placed adjacent to the slices to selectively destroy MR signal from blood flowing in from one side of the slice. This allows the technique to be flow direction sensitive and useful for creating arteriograms or venograms. TOF MRA is acquired with sequential 2DFT slices, 3DFT slabs or multiple overlapping thin 3D FT slabs (MOTSA) depending on the coverage required and the range of flow velocities under examination. The images can be processed by Maximum Intensity Projection (MIP) to interactively create projection angiograms is a range of views. For complete interpretation the base slices should also be reviewed individually and with multi-planar reconstruction (MPR) software.
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    Applications of TOF MRA


    Limitations of TOF MRA
  • Must be targeted to a specific flow scenario or clinical condition
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  • Phase Contrast Angiography (PCA)

    During an MRI sequence, spatially varying or gradient magnetic fields are applied to spatially localize signal. The method assumes that everything remains stationary. If signal producing tissue (like blood) moves significantly during the sequence the spatial phase encoding is incorrect and a ghost image of the object will be seen mis-mapped in the phase encoded direction. The amount of phase error depends on the velocity of the movement and the strength and period of the applied gradient. In a motion compensated sequence these artefacts are avoided.

    The PCA method acquires two sequences; one with and one without motion compensation. By comparing the phase of signals from each location in the two sequences the exact amount of motion induced phase change can be determined so we have a map where pixel brightness is proportional to spatial velocity. Regions that are stationary remain black while moving regions are represented as grey to white.

    In its basic form the PCA sequence only shows a signal where a voxel contains uniform velocity in a specific direction. By acquiring 3 sequences each with motion compensation in the three orthogonal directions a complete map of flow regardless of direction is obtained as well as the motion.

    Phase change is measured on a circular scale, 0,1,2358,359,0,1 degrees. PCA sequences are designed so different flow velocities display the maximum 360 degrees of phase shift. This factor is called the velocity Encoding factor or VENC. For best results the VENC is chosen to be slightly more than the maximum expected flow velocity in a region. If a higher velocity is encountered it will be represented as a darker pixel. This is known as velocity aliasing.


    PCA Implementations

    2D PCA

    A thick slice 3 direction PCA can generate a single projection angiogram in about 1.5 minutes. The method is commonly used to rapidly determine cerebral vein patency, identify venous angiomas and simple AVM, test the appropriate VENC before running a long 3DFT PCA, or provide vascular scout images to accurately position other MRA sequences.

    3D PCA

    A 3DFT technique is used to partition a thick slab PCA sequence into thin slices which can be processed in MIP and MPR programmes to create multiple projection angiograms. A 3D PCA takes about twice as long to perform as a comparable TOF MRA but is affected by different artefacts and much more sensitive to flow at lower velocities.

    2D Cine PCA

    A cardiac triggered version of the 2D PCA sequence provides a number of views of the flow at different stages of the cardiac cycle. It is particularly used for pulsatile flow of blood and CSF. The technique uses long sequences and relies on a regular heart rate.
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    PCA Applications

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    When is MRA Appropriate?


    Summary

    With appropriate technique, conventional MRA offers accurate vascular information with no risk to the patient in a plainly understandable format. It can provide quick effective display of cerebral vessels, neck vessels beyond the reach of ultrasound, and the peripheral circulation. Its ability to display vessels is limited by spatial resolution particularly the slice thickness. Availability of MR scanners, the speed and capability of current hardware, and acceptance limit its application by clinicians. MRA remains an exciting technique because the latter points are changing rapidly.

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    MRA Flow Quantification and Contrast Enhanced MRA

    Each of these techniques can be used to extend the role of MRA beyond that available with conventional TOF and PCA. Their current implementation as practical clinical tools stem from being able to complete the scan sequences within a single breath-hold period. This is possible because of the high performance gradient systems and phased array coils that are only recently achieving broad distribution.

    Flow quantification by MR using breath hold sequences offers the rapid collection of functional vascular information and should alter the role of MRA as significantly as the addition of Doppler techniques did to vascular ultrasound. CE-MRA is being heralded as the technique that will handle the previously marginal task of abdominal MRA, positioning MRA to seriously challenge DSA as the primary vascular imaging modality. 

    Flow Quantification (MR-FQ)

    The addition of a quantitative analysis of flow rates and time varying blood velocity is used to enhance the value of the MRA examination. MR FQ uses a cardiac triggered 2DFT phase contrast technique. The images can be examined and regions of interest placed over target vessels to generate velocity/time graphs. If the ROI covers the entire vessel, peak and mean flow values can be determined accurately.

    Fine temporal resolution is essential to ensure accurate representation of highly pulsatile flow. Conventional MR-FQ sequence times of three minutes make the method susceptible to errors based on heart rate variation and respiratory motion, however they can achieve temporal resolutions of 20-50 mSec. Breath hold sequences offer good results but the temporal resolution may fall to 150 mSec. This can be countered by repeated scans with variable ECG trigger delays as long as the patient can breath hold consistently.
     
     
    Effects of Sampling Rate on Curve Representation

    Measures of mean flow volumes are most sensitive to inaccuracies in the time/velocity curve as this figure is calculated from the integral of the curve. (area under the curve). The mean flow figures are used to establish if a stenosis actually reduces tissue perfusion (haemodynamic effect). Values can be obtained proximal and distal to the stenosis, or by comparing down stream values to known standards.


    Applications MR-FQ is commonly used in larger relatively stationary vessels such as the Aorta, Iliac, and Carotid arteries. Its application in renal artery stenosis has been reported but is difficult to reproduce without breath-hold scans.
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    Contrast Enhanced MRA (CE- MRA, Gd- MRA)

    CE-MRA is almost directly analogous to IV-DSA and IV-CTA. An intra-venous injection of a short dense bolus of a gadolinium based contrast agent is imaged on the first pass through the arterial system. Sequential scans are added to acquire early and late venous images. Further delayed sequences will record an MR Urogram much like IVP delayed images if required. CE-MRA images are essentially a record of the vessel lumen. Some flow mechanisms affect the contrast of these images, but the sequence parameters are chosen to minimize them. The gadolinium is being used as a blood pool agent to be imaged as it transits the region of interest, although it is not the actual contrast agent that is visible on the images. In high concentrations, the Gd radically shortens the T1 of the adjacent blood. The extremely short TR values (3-7 mSec) ensure saturation of almost all stationary tissues and a high sensitivity to the short T1 gadolinium enhanced blood. Fat signal can remain in the images but these are removed by subtraction. The short TE maximizes signal and minimizes pulsatile ghosts and signal loss from regions of chaotic or non-streamline flow.

    The CE-MRA method has been used since about 1993 but it has become reliable and more robust with the advent of breath-hold sequences. These sequences rely on the use of extremely fast sequences and high performance gradient and coil systems. For those reasons the CE-MRA sequences have only been commercially available on high end MR systems since late 1996. Many investigators are working to define the technique's clinical role.

    The technique described later in this paper is currently in use at Royal Adelaide Hospital MRI Unit. It is closely based on the method suggested in the Siemens MAGNETOM Application Guide.

    CE-MRA Advantages

    CE-MRA Disadvantages & Pitfalls

    CE-MRA Applications

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    CE-MRA Technique

    Patient Preparation

    Sequence Contrast Injection
    MAGNAVIST (Gadopentatate, Schering) is currently used but equal results have been reported using OMNISCAN (Gadodiamide, Nycomed). A contrast dose between 0.3 and 0.6 ml./kg (0.15 - 0.3 mmol/kg) produces acceptable results. All patients over 50 kg receive a 29 ml. bolus injected at approximately 3 ml./sec. per second for the main imaging run. The contrast bolus is followed immediately with a 30 ml. bolus of saline injected at the same rate.

    Scan Timing
    Proper co-ordination of the scan sequence and injection is vital to the quality of CE-MRA, particularly when using low Gd doses (0.2-0.3 ml/kg). Most of the image contrast is derived during the acquisition of the central ½ of k-space, (7-21 seconds after scan start), so the contrast bolus must be in the field of view during this period.

    Timing Run & Establishing Transit Time:

    Planning and team work Tips Image Handling top of page

    Suggested Reading

    MRI Physics of Flow and MRA. Evan Fram Book of Abstracts the Gold Coast MRI & CT Conference. 1996

    SIGNA Applications Guide Volume 3. P.Turski (editor) GE Medical Systems. 1990

    MAGNETOM Applications Guide 1 Techniques Section D2. Siemens Medical Systems 1997



    References
    Renal Artery Stenosis: Morphologic & Functional Evaluation by Gadolinium Enhanced MR. Schoenberg, Knapp, Bock, Prince et al Abstract 122 Proceedings International Society of Magnetic Resonance in Medicine March 1997
    MR Contrast Urography by Breath-Hold Contrast Enhanced Three Dimensional FISP Li W, Chavez D, Edelman R, Potturmarthi, Prasad V. JMRI  1997 7:309-311
    Prince M. Yucel E., Kaufmann J.,Harrison D., Geller S Dynamic Gadolinium-enhanced Three Dimensional Abdominal MR Arteriography JMRI 1993 3:877-881
    Debatin J., Hany T., any T. Ha  Progress in MR Angiography Insert Cardiovascular & Interventional Radiology Vol.20 Issue 1 January 1997
    Optimized Breathhold 3D MRA A.Shetty K.Bis R Loretitsch FLASH V5 No.2 P4-5 June 1997


    Acknowledgements
    Dr James Taylor   Director of MRI Royal Adelaide Hospital
    Trina White          MR Product Specialist. Medical Applications Pty Ltd
    Anil Gupta            MR Applications Specialist. Medical Applications Pty Ltd
    Joanna Rentis Schering Australia
    Su Collings          Nycomed Australia
    The Radiographers of the Royal Adelaide Hospital MRI Unit.

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    If you would like this paper presented at your MRI meeting or you have comments or suggestions, please contact the author by e-mail. 
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